Object Classes

FastqcData()

The FastqcData Object Class

FastqcDataList()

The FastqcDataList Object Class

FastpData()

The FastpData Object Class

FastpDataList()

The FastpDataList Object Class

PwfCols-class PwfCols

The PwfCols class and associated methods

TheoreticalGC-class

The TheoreticalGC Object Class

Working With FastQC or Fastp Reports

getModule(<FastqcData>) getModule(<FastqcDataList>) getModule(<ANY>) getModule(<FastpData>) getModule(<FastpDataList>)

Retrieve a given module from a Fastqc* Object

getSummary(<.FastqcFile>) getSummary(<ANY>) getSummary(<FastqcData>) getSummary(<FastqcDataList>)

Get the summary information from Fastqc Files

plotReadTotals()

Draw a barplot of read totals

readTotals()

Get the read totals

plotSummary()

Plot the PASS/WARN/FAIL information

plotAdapterContent()

Draw an Adapter Content Plot

plotBaseQuals()

Plot the Base Qualities for each file

plotDupLevels()

Plot the combined Sequence_Duplication_Levels information

plotGcContent()

Plot the Per Sequence GC Content

plotInsertSize()

Plot Insert Size Distributions

plotKmers()

Plot Overrepresented Kmers

plotNContent()

Draw an N Content Plot

plotOverrep()

Plot a summary of Over-represented Sequences

summariseOverrep()

Summarise Overrepresented Sequences

plotFastqcPCA()

Draw a PCA plot for Fast QC modules

plotSeqContent()

Plot the per base content as a heatmap

plotSeqLengthDistn()

Plot the Sequence Length Distribution

plotSeqQuals()

Plot the Per Sequence Quality Scores

writeHtmlReport()

Write an HTML Summary Report

maxAdapterContent()

Get the maximum Adapter Content

fqName() `fqName<-`()

Return the Underlying Fastq File Names from Fastqc/Fastp Objects

fqcVersion(<FastqcData>) fqcVersion(<FastqcDataList>) fqcVersion(<ANY>)

Get the FASTQC version

Working With Other NGS Tools

importNgsLogs()

Import Various NGS-related log files

importSJ()

Import STAR Splice Junctions

plotAlignmentSummary()

Plot a summary of alignments

plotAssemblyStats()

Plot a summary of assembly logs

Utility Functions

estGcDistn()

Estimate a GC Content Distribution From Sequences

gcAvail()

List Genomes or Transcriptomes with Theoretical GC Content

getGC()

Get Theoretical GC content

getColours(<PwfCols>) setColours(<PwfCols>) setAlpha(<PwfCols>)

Work with objects of class PwfCols

overRep2Fasta()

Write fasta of Over-Represented sequences.

isCompressed()

Check to see if a file is compressed

path(<.FastqcFile>) path(<FastqcData>) path(<FastqcDataList>) path(<.FastpFile>) path(<FastpData>) path(<FastpDataList>)

Return the File Paths from an object

mData()

Extract Metadata for TheoreticalGC objects

Data Objects

gcTheoretical

Theoretical GC content

pwf

Colours for PASS/WARN/FAIL

All Methods

estGcDistn()

Estimate a GC Content Distribution From Sequences

`[`(<FastqcDataList>,<numeric>,<missing>) `[`(<FastqcDataList>,<character>,<missing>) `[`(<FastqcDataList>,<logical>,<missing>) `[`(<FastqcDataList>,<ANY>,<missing>) `[`(<FastpDataList>,<numeric>,<missing>) `[`(<FastpDataList>,<character>,<missing>) `[`(<FastpDataList>,<logical>,<missing>) `[`(<FastpDataList>,<ANY>,<missing>)

Extract Elements

fqName() `fqName<-`()

Return the Underlying Fastq File Names from Fastqc/Fastp Objects

fqcVersion(<FastqcData>) fqcVersion(<FastqcDataList>) fqcVersion(<ANY>)

Get the FASTQC version

gcAvail()

List Genomes or Transcriptomes with Theoretical GC Content

getColours(<PwfCols>) setColours(<PwfCols>) setAlpha(<PwfCols>)

Work with objects of class PwfCols

getGC()

Get Theoretical GC content

getModule(<FastqcData>) getModule(<FastqcDataList>) getModule(<ANY>) getModule(<FastpData>) getModule(<FastpDataList>)

Retrieve a given module from a Fastqc* Object

getSummary(<.FastqcFile>) getSummary(<ANY>) getSummary(<FastqcData>) getSummary(<FastqcDataList>)

Get the summary information from Fastqc Files

mData()

Extract Metadata for TheoreticalGC objects

overRep2Fasta()

Write fasta of Over-Represented sequences.

path(<.FastqcFile>) path(<FastqcData>) path(<FastqcDataList>) path(<.FastpFile>) path(<FastpData>) path(<FastpDataList>)

Return the File Paths from an object

plotAdapterContent()

Draw an Adapter Content Plot

plotBaseQuals()

Plot the Base Qualities for each file

plotDupLevels()

Plot the combined Sequence_Duplication_Levels information

plotFastqcPCA()

Draw a PCA plot for Fast QC modules

plotGcContent()

Plot the Per Sequence GC Content

plotInsertSize()

Plot Insert Size Distributions

plotKmers()

Plot Overrepresented Kmers

plotNContent()

Draw an N Content Plot

plotOverrep()

Plot a summary of Over-represented Sequences

plotReadTotals()

Draw a barplot of read totals

plotSeqContent()

Plot the per base content as a heatmap

plotSeqLengthDistn()

Plot the Sequence Length Distribution

plotSeqQuals()

Plot the Per Sequence Quality Scores

plotSummary()

Plot the PASS/WARN/FAIL information

summariseOverrep()

Summarise Overrepresented Sequences